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人流行性乙型腦炎抗體IgG酶免試劑盒,(JE IgG)ELISA檢測試劑盒,英文名:JE IgG ELISA Kit,英文縮寫:JE IgG ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人流行性乙型腦炎抗體IgGELISA檢測試劑盒,JE IgG 檢測試劑盒,JE IgG 檢測試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。人流行性乙型腦炎抗體IgG酶免試劑盒,(JE IgG)ELISA檢測試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競爭法及定量檢測影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
白介素21(IL21)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 21 (IL21)48T/96TY09885Homo sapiens (Human)
白介素28A(IL28A)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 28A (IL28A)48T/96TY09886Homo sapiens (Human)
滲透性糖蛋白(Pgp)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Permeability Glycoprotein (Pgp)48T/96TY09887Homo sapiens (Human)
角蛋白10(KRT10)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Keratin 10 (KRT10)48T/96TY09888Homo sapiens (Human)
胸腺激活調(diào)節(jié)趨化因子(TARC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Thymus Activation Regulated Chemokine (TARC)48T/96TY09889Rattus norvegicus (Rat)
脂肪細(xì)胞型脂肪酸結(jié)合蛋白(FABP4)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fatty Acid Binding Protein 4, Adipocyte (FABP4)48T/96TY09890Homo sapiens (Human)
白介素25(IL25)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 25 (IL25)48T/96TY09891Homo sapiens (Human)
白介素26(IL26)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 26 (IL26)48T/96TY09892Homo sapiens (Human)
血管內(nèi)皮生長因子165(VEGF165)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Vascular Endothelial Growth Factor 165 (VEGF165)48T/96TY09893Homo sapiens (Human)
α1-抗胰蛋白酶(α1AT)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Alpha-1-Antitrypsin (a1AT)48T/96TY09894Homo sapiens (Human)
乳酸脫氫酶B(LDHB)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Lactate Dehydrogenase B (LDHB)48T/96TY09895Homo sapiens (Human)
乳酸脫氫酶B(LDHB)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Lactate Dehydrogenase B (LDHB)48T/96TY09896Rattus norvegicus (Rat)
乳酸脫氫酶B(LDHB)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Lactate Dehydrogenase B (LDHB)48T/96TY09897Mus musculus (Mouse)
血管生成素樣蛋白3(ANGPTL3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Angiopoietin Like Protein 3 (ANGPTL3)48T/96TY09898Homo sapiens (Human)
白介素17B(IL17B)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 17B (IL17B)48T/96TY09899Rattus norvegicus (Rat)
WAP四二硫化物核心域蛋白1(WFDC1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for WAP Four Disulfide Core Domain Protein 1 (WFDC1)48T/96TY09900Mus musculus (Mouse)
胰淀素(IAPP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Islet Amyloid Polypeptide (IAPP)48T/96TY09901Oryctolagus cuniculus (Rabbit)
核糖核酸酶A3(RNASE3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Ribonuclease A3 (RNASE3)48T/96TY09902Homo sapiens (Human)
TATA盒結(jié)合蛋白(TBP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for TATA Binding Protein (TBP)48T/96TY09903Homo sapiens (Human)
腫瘤壞死因子α(TNFα)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY09904Canis familiaris; Canine (Dog)
粒細(xì)胞巨噬細(xì)胞集落刺激因子(GMCSF)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)48T/96TY09905Canis familiaris; Canine (Dog)
髓分化因子88(MyD88)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Myeloid Differentiation Factor 88 (MyD88)48T/96TY09906Homo sapiens (Human)
法尼基二磷酸法尼基轉(zhuǎn)移酶1(FDFT1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Farnesyl Diphosphate Farnesyltransferase 1 (FDFT1)48T/96TY09907Homo sapiens (Human)
T-細(xì)胞白血病/淋巴瘤蛋白1A(TCL1A)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for T-Cell Leukemia/Lymphoma Protein 1A (TCL1A)48T/96TY09908Homo sapiens (Human)
糖盞蛋白(GC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glycocalicin (GC)48T/96TY09909Homo sapiens (Human)
Kruppel樣因子5(KLF5)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Kruppel Like Factor 5, Intestinal (KLF5)48T/96TY09910Homo sapiens (Human)
卷曲同源物8(FZD8)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Frizzled Homolog 8 (FZD8)48T/96TY09911Homo sapiens (Human)
信號(hào)傳導(dǎo)轉(zhuǎn)錄激活因子1(STAT1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Signal Transducer And Activator Of Transcription 1 (STAT1)48T/96TY09912Homo sapiens (Human)
轉(zhuǎn)化生長因子β3(TGFβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)48T/96TY09913Sus scrofa; Porcine (Pig)
蛋白激酶Bβ(PKBβ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Protein Kinase B Beta (PKBb)48T/96TY09914Homo sapiens (Human)
白介素2受體α(IL2Rα)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 2 Receptor Alpha (IL2Ra)48T/96TY09915Sus scrofa; Porcine (Pig)
絲裂原激活蛋白激酶激酶6(MAP2K6)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Mitogen Activated Protein Kinase Kinase 6 (MAP2K6)48T/96TY09916Homo sapiens (Human)
血清/糖皮質(zhì)激素調(diào)節(jié)激酶2(SGK2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Serum/Glucocorticoid Regulated Kinase 2 (SGK2)48T/96TY09917Homo sapiens (Human)
表面活性物質(zhì)關(guān)聯(lián)蛋白D(SPD)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Surfactant Associated Protein D (SPD)48T/96TY09918Rattus norvegicus (Rat)
吡哆醛激酶(PDXK)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Pyridoxal Kinase (PDXK)48T/96TY09919Homo sapiens (Human)
CD163分子(CD163)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cluster Of Differentiation (CD163)48T/96TY09920Homo sapiens (Human)
信號(hào)傳導(dǎo)轉(zhuǎn)錄激活因子5B(STAT5B)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Signal Transducer And Activator Of Transcription 5B (STAT5B)48T/96TY09921Homo sapiens (Human)
生長分化因子2(GDF2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Growth Differentiation Factor 2 (GDF2)48T/96TY09922Homo sapiens (Human)
癌蛋白誘導(dǎo)轉(zhuǎn)錄物3(OIT3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Oncoprotein Induced Transcript 3 (OIT3)48T/96TY09923Homo sapiens (Human)
B-細(xì)胞淋巴瘤因子3(Bcl3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for B-Cell CLL/Lymphoma 3 (Bcl3)48T/96TY09924Homo sapiens (Human)
胱天蛋白酶4(CASP4)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Caspase 4 (CASP4)48T/96TY09925Homo sapiens (Human)
激活STAT蛋白抑制因子1(PIAS1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1)48T/96TY09926Homo sapiens (Human)
信號(hào)傳導(dǎo)轉(zhuǎn)錄激活因子3(STAT3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Signal Transducer And Activator Of Transcription 3 (STAT3)48T/96TY09927Homo sapiens (Human)
泛素D(UBD)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Ubiquitin D (UBD)48T/96TY09928Homo sapiens (Human)
血小板型磷酸果糖激酶(PFKP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Phosphofructokinase, Plaet (PFKP)48T/96TY09929Homo sapiens (Human)
己糖激酶3(HK3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Hexokinase 3, White Cell (HK3)48T/96TY09930Homo sapiens (Human)
色氨酰tRNA合成酶(WARS)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tryptophanyl tRNA Synthetase (WARS)48T/96TY09931Homo sapiens (Human)
二氫嘧啶酶樣蛋白3(DPYSL3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Dihydropyrimidinase Like Protein 3 (DPYSL3)48T/96TY09932Homo sapiens (Human)
腫瘤壞死因子配體超家族成員13(TNFSF13)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13)48T/96TY09933Homo sapiens (Human)
接觸蛋白4(CNTN4)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Contactin 4 (CNTN4)48T/96TY09934Rattus norvegicus (Rat)
各種類型ELISA測定時(shí),一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。